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Product Description
Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit | ELK5551 | ELK Biotechnology
Alternative Names: AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 0.94 μg/mL
Standard: 200 μg/mL
Detection Range: 3.13-200 μg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Infection immunity;Immune molecule;Kidney biomarker
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-1-Microglobulin/Bikunin Precursor (a1M) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Microglobulin/Bikunin Precursor (a1M) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Microglobulin/Bikunin Precursor (a1M) in the samples is then determined by comparing the OD of the samples to the standard curve.
