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Product Description
Mouse Dyn (Big Dynorphin) ELISA Kit | ELK2453 | ELK Biotechnology
Alternative Names: B-DYN; Biocytin 13 Dynorphin A 1-13 Amide
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 1.35 pg/mL
Standard: 300 pg/mL
Detection Range: 4.69-300 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 2h
Research Area: Signal transduction;Neuro science
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Big Dynorphin (Dyn) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Big Dynorphin (Dyn) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Big Dynorphin (Dyn) in the samples is then determined by comparing the OD of the samples to the standard curve.
