Euro
USD
British Pound
NULL
Product Description
Mouse GT (Gastrin) ELISA Kit | ELK2706 | ELK Biotechnology
Alternative Names: GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 4.77 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gastrin (GT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gastrin (GT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gastrin (GT) in the samples is then determined by comparing the OD of the samples to the standard curve.
