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Product Description
Mouse KLK1 (Kallikrein 1) ELISA Kit | ELK4995 | ELK Biotechnology
Alternative Names: KLKR; Klk6; HK1; Kallikrein 1, Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 19.1 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 2h
Research Area: Enzyme & Kinase;Tumor immunity;Hepatology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 1 (KLK1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 1 (KLK1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 1 (KLK1) in the samples is then determined by comparing the OD of the samples to the standard curve.
