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Product Description
Mouse NES1 (Nesfatin 1) ELISA Kit | ELK5437 | ELK Biotechnology
Alternative Names: Nesfatin-1
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 191.7 pg/mL
Standard: 40000 pg/mL
Detection Range: 625-40000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Glycolipid metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Nesfatin 1 (NES1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nesfatin 1 (NES1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nesfatin 1 (NES1) in the samples is then determined by comparing the OD of the samples to the standard curve.
