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Product Description
Mouse PINP (Procollagen I N-Terminal Propeptide) ELISA Kit | ELK5403 | ELK Biotechnology
Alternative Names: P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 152.5 pg/mL
Standard: 30000 pg/mL
Detection Range: 468.75-30000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Hepatology;Bone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Procollagen I N-Terminal Propeptide (PINP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen I N-Terminal Propeptide (PINP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the OD of the samples to the standard curve.
