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Product Description
Mouse UCN2 (Urocortin 2) ELISA Kit | ELK5581 | ELK Biotechnology
Alternative Names: URP; SRP; UCN-II; UCNII; Stresscopin-Related Peptide; Urocortin-related peptide
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 0.44 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Urocortin 2 (UCN2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Urocortin 2 (UCN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Urocortin 2 (UCN2) in the samples is then determined by comparing the OD of the samples to the standard curve.
