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Product Description
Mouse Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit | ELK4915 | ELK Biotechnology
Alternative Names: Amyloid beta 42 (ABeta 42) ; amyloid beta peptide 1-42; Aβ1-42, amyloid βpeptide 1-42
Species: Mouse
Assay Type: Sandwich
Sensitivity: 2.64 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amyloid Beta Peptide 1-42 (Ab1-42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the samples is then determined by comparing the OD of the samples to the standard curve.
