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Product Description
Mouse APOH (Apolipoprotein H) ELISA Kit | ELK4804 | ELK Biotechnology
Alternative Names: Apo-H; B2G1; BG; B2-GP1; B2GP1; Previously; Beta 2 Glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Anticardiolipin cofactor; Beta (2) GPI
Species: Mouse
Assay Type: Sandwich
Sensitivity: 1.42 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma, saliva and other biological fluids.
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein H (APOH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein H (APOH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein H (APOH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein H (APOH) in the samples is then determined by comparing the OD of the samples to the standard curve.
