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Product Description
Mouse CACNa1H (Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit) ELISA Kit | ELK6876 | ELK Biotechnology
Alternative Names: CACNA1HB; Cav3.2; Low-voltage-activated calcium channel alpha1 3.2 subunit; Voltage-gated calcium channel subunit alpha Cav3.2
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.117 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H) in the samples is then determined by comparing the OD of the samples to the standard curve.
