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Product Description
Mouse CACNa1S (Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit) ELISA Kit | ELK6870 | ELK Biotechnology
Alternative Names: CACNL1A3; CCHL1A3; Cav1.1; HOKPP; MHS5; hypoPP; DHPR; Dihydropyridine Receptor; Voltage-gated calcium channel subunit alpha Cav1.1
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.101 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S) in the samples is then determined by comparing the OD of the samples to the standard curve.
