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Product Description
Mouse CCKAR (Cholecystokinin A Receptor) ELISA Kit | ELK2294 | ELK Biotechnology
Alternative Names: CCK-A; CCK1; CCKRA; CCK1R; Cholecystokinin Type 1 Receptor
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Endocrinology;Hepatology;Hormone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholecystokinin A Receptor (CCKAR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholecystokinin A Receptor (CCKAR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholecystokinin A Receptor (CCKAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholecystokinin A Receptor (CCKAR) in the samples is then determined by comparing the OD of the samples to the standard curve.
