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Product Description
Mouse CD59 (Protectin) ELISA Kit | ELK4756 | ELK Biotechnology
Alternative Names: MIC11; MIN1; MIN2; MIN3; MSK21; MAC-IP; MACIF; MIRL; HRF20; Complement Regulatory Protein; 20 kDa homologous restriction factor; Membrane attack complex inhibition factor
Species: Mouse
Assay Type: Sandwich
Sensitivity: 25.5 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma, urine and other biological fluids.
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Hematology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protectin (CD59) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protectin (CD59) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protectin (CD59), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protectin (CD59) in the samples is then determined by comparing the OD of the samples to the standard curve.
