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Product Description
Mouse CHRM3 (Cholinergic Receptor, Muscarinic 3) ELISA Kit | ELK4283 | ELK Biotechnology
Alternative Names: CHR-M3; HM3; M-AChRM3; Muscarinic Acetylcholine Receptor M3; Cholinergic Receptor, Muscarinic 3
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Muscarinic 3 (CHRM3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholinergic Receptor, Muscarinic 3 (CHRM3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Muscarinic 3 (CHRM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Muscarinic 3 (CHRM3) in the samples is then determined by comparing the OD of the samples to the standard curve.
