Euro
USD
British Pound
NULL
Product Description
Mouse CTSB (Cathepsin B) ELISA Kit | ELK7100 | ELK Biotechnology
Alternative Names: CTS-B; APPS; CPSB; APP secretase
Species: Mouse
Assay Type: Sandwich
Sensitivity: 12.2 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin B (CTSB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cathepsin B (CTSB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin B (CTSB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin B (CTSB) in the samples is then determined by comparing the OD of the samples to the standard curve.
