Product Description
Mouse DKK4 (Dickkopf Related Protein 4) ELISA Kit | ELK7947 | ELK Biotechnology
Alternative Names: Dickkopf 4; Dickkopf gene 4; Dickkopf-4; DKK 4; Dkk-4; DKK4
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.051 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 4 (DKK4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dickkopf Related Protein 4 (DKK4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 4 (DKK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dickkopf Related Protein 4 (DKK4) in the samples is then determined by comparing the OD of the samples to the standard curve.