Euro
USD
British Pound
NULL
Product Description
Mouse ELA2 (Elastase 2, Neutrophil) ELISA Kit | ELK2332 | ELK Biotechnology
Alternative Names: ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.062 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 2, Neutrophil (ELA2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Elastase 2, Neutrophil (ELA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 2, Neutrophil (ELA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 2, Neutrophil (ELA2) in the samples is then determined by comparing the OD of the samples to the standard curve.
