Euro
USD
British Pound
NULL
Product Description
Mouse ERb (Estrogen Receptor Beta) ELISA Kit | ELK4281 | ELK Biotechnology
Alternative Names: ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3, Group A, Member 2
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.142 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Beta (ERb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Beta (ERb) in the samples is then determined by comparing the OD of the samples to the standard curve.
