Euro
USD
British Pound
NULL
Product Description
Mouse FGF7 (Fibroblast Growth Factor 7) ELISA Kit | ELK3081 | ELK Biotechnology
Alternative Names: KGF; HBGF7; Keratinocyte Growth Factor; Heparin-binding growth factor 7
Species: Mouse
Assay Type: Sandwich
Sensitivity: 2.8 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Developmental science;Pulmonology;Dermatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 7 (FGF7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibroblast Growth Factor 7 (FGF7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 7 (FGF7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 7 (FGF7) in the samples is then determined by comparing the OD of the samples to the standard curve.
