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Product Description
Mouse GP73 (Golgi Protein 73) ELISA Kit | ELK3906 | ELK Biotechnology
Alternative Names: GOLM1; GOLPH2; C9orf155; Golgi Membrane Protein 1; Golgi Phosphoprotein 2
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.37 ng/mL
Standard: 60 ng/mL
Detection Range: 0.94-60 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity;Reproductive science;Hepatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi Protein 73 (GP73) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Golgi Protein 73 (GP73) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Golgi Protein 73 (GP73), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Golgi Protein 73 (GP73) in the samples is then determined by comparing the OD of the samples to the standard curve.
