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Product Description
Mouse HSD17b10 (17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit | ELK6973 | ELK Biotechnology
Alternative Names: 17b-HSD10; ABAD; ERAB; HADH2; HCD2; MHBD; SCHAD; SDR5C1, MRPP2; CAMR; 3-Hydroxyacyl-CoA Dehydrogenase Type-2; AB-Binding Alcohol Dehydrogenase
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) in the samples is then determined by comparing the OD of the samples to the standard curve.
