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Product Description
Mouse IL5Ra (Interleukin 5 Receptor Alpha) ELISA Kit | ELK3714 | ELK Biotechnology
Alternative Names: CDw125; CD125; IL5R-A; IL5-RA; HSIL5R3; IL5R; IL5R-A;IL-5RA
Species: Mouse
Assay Type: Sandwich
Sensitivity: 28.5 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 Receptor Alpha (IL5Ra) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 5 Receptor Alpha (IL5Ra) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 5 Receptor Alpha (IL5Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 5 Receptor Alpha (IL5Ra) in the samples is then determined by comparing the OD of the samples to the standard curve.
