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Product Description
Mouse MTOR (Serine/threonine-protein kinase mTOR) ELISA Kit | ELK8644 | ELK Biotechnology
Alternative Names: FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; FK506 Binding Protein 12 Rapamycin Associated Protein; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cell proliferation
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/threonine-protein kinase mTOR (MTOR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serine/threonine-protein kinase mTOR (MTOR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/threonine-protein kinase mTOR (MTOR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/threonine-protein kinase mTOR (MTOR) in the samples is then determined by comparing the OD of the samples to the standard curve.
