Euro
USD
British Pound
NULL
Product Description
Mouse MUC4 (Mucin 4) ELISA Kit | ELK3337 | ELK Biotechnology
Alternative Names: ASGP; Mucin 4 Cell Surface Associated; Mucin 4, Tracheobronchial; Testis mucin; Ascites sialoglycoprotein; Pancreatic adenocarcinoma mucin
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.26 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity;Pulmonology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 4 (MUC4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mucin 4 (MUC4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 4 (MUC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 4 (MUC4) in the samples is then determined by comparing the OD of the samples to the standard curve.
