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Product Description
Mouse NEUROG3 (Neurogenin 3) ELISA Kit | ELK6561 | ELK Biotechnology
Alternative Names: Math4B; Atoh5; ngn3; bHLHa7; Class A basic helix-loop-helix protein 7; Protein atonal homolog 5
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurogenin 3 (NEUROG3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurogenin 3 (NEUROG3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurogenin 3 (NEUROG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurogenin 3 (NEUROG3) in the samples is then determined by comparing the OD of the samples to the standard curve.
