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Product Description
Mouse NPY (Neuropeptide Y) ELISA Kit | ELK2449 | ELK Biotechnology
Alternative Names: NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.97 pg/mL
Standard: 200 pg/mL
Detection Range: 3.13-200 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hepatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide Y (NPY) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuropeptide Y (NPY) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropeptide Y (NPY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropeptide Y (NPY) in the samples is then determined by comparing the OD of the samples to the standard curve.
