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Product Description
Mouse PDIA4 (Protein Disulfide Isomerase A4) ELISA Kit | ELK6948 | ELK Biotechnology
Alternative Names: PDI-A4; ERP70; ERP72; Protein Disulfide Isomerase-Associated 4; Endoplasmic reticulum resident protein 70
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.112 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A4 (PDIA4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A4 (PDIA4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A4 (PDIA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A4 (PDIA4) in the samples is then determined by comparing the OD of the samples to the standard curve.
