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Product Description
Mouse PIGR (Polymeric Immunoglobulin Receptor) ELISA Kit | ELK3609 | ELK Biotechnology
Alternative Names: SC; Secretory Component; Hepatocellular carcinoma-associated protein TB6
Species: Mouse
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Infection immunity;Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymeric Immunoglobulin Receptor (PIGR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Polymeric Immunoglobulin Receptor (PIGR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymeric Immunoglobulin Receptor (PIGR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymeric Immunoglobulin Receptor (PIGR) in the samples is then determined by comparing the OD of the samples to the standard curve.
