Euro
USD
British Pound
NULL
Product Description
Mouse REG1a (Regenerating Islet Derived Protein 1 Alpha) ELISA Kit | ELK4869 | ELK Biotechnology
Alternative Names: REG; PSP; ICRF; PSPS1; PTP; Islet cells regeneration factor; Islet of Langerhans regenerating protein; Lithostathine 1 Alpha; Pancreatic Stone Protein; Pancreatic Thread Protein
Species: Mouse
Assay Type: Sandwich
Sensitivity: 3.6 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Infection immunity;Endocrinology;Hepatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 1 Alpha (REG1a) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 1 Alpha (REG1a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 1 Alpha (REG1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 1 Alpha (REG1a) in the samples is then determined by comparing the OD of the samples to the standard curve.
