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Product Description
Mouse S100B (S100 Calcium Binding Protein B) ELISA Kit | ELK2825 | ELK Biotechnology
Alternative Names: S100-B; NEF; S100Beta; S-100 protein subunit beta; S100 protein beta chain; Soluble protein-100B; Soluble protein 100B
Species: Mouse
Assay Type: Sandwich
Sensitivity: 12.5 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein B (S100B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S100 Calcium Binding Protein B (S100B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein B (S100B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein B (S100B) in the samples is then determined by comparing the OD of the samples to the standard curve.
