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Product Description
Mouse S1PR5 (Sphingosine 1 Phosphate Receptor 5) ELISA Kit | ELK7432 | ELK Biotechnology
Alternative Names: EDG8; S1P5; S1PR5; SPPR-1; SPPR-2; Endothelial Differentiation, Sphingolipid G-Protein-Coupled Receptor, 8; Sphingosine 1-phosphate receptor Edg-8
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 5 (S1PR5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 5 (S1PR5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 5 (S1PR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 5 (S1PR5) in the samples is then determined by comparing the OD of the samples to the standard curve.
