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Product Description
Mouse SCCA2 (Squamous Cell Carcinoma Antigen 2) ELISA Kit | ELK2847 | ELK Biotechnology
Alternative Names: SERPINB4; PI11; SPB4; Leupin; Serpin Peptidase Inhibitor, Clade B, Member 4; Peptidase Inhibitor 11
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity;Gastroenterology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Squamous Cell Carcinoma Antigen 2 (SCCA2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Squamous Cell Carcinoma Antigen 2 (SCCA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Squamous Cell Carcinoma Antigen 2 (SCCA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Squamous Cell Carcinoma Antigen 2 (SCCA2) in the samples is then determined by comparing the OD of the samples to the standard curve.
