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Product Description
Mouse SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit | ELK6778 | ELK Biotechnology
Alternative Names: FRP-2; SARP1; SDF-5; Secreted apoptosis-related protein 1
Species: Mouse
Assay Type: Sandwich
Sensitivity: 46 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 2 (SFRP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 2 (SFRP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 2 (SFRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 2 (SFRP2) in the samples is then determined by comparing the OD of the samples to the standard curve.
