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Product Description
Mouse WNT4 (Wingless Type MMTV Integration Site Family, Member 4) ELISA Kit | ELK8130 | ELK Biotechnology
Alternative Names: MGC123964; Protein Wnt-4; RP23-246F18.1; SERKAL; WNT 4; WNT 4 protein precursor; WNT4
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 4 (WNT4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 4 (WNT4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 4 (WNT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 4 (WNT4) in the samples is then determined by comparing the OD of the samples to the standard curve.
