Product Description
Pig AGC (Aggrecan) ELISA Kit | ELK9048 | ELK Biotechnology
Alternative Names: ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1; Aggrecan core protein
Species: Pig
Assay Type: Sandwich
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Metabolic pathway;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aggrecan (AGC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aggrecan (AGC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aggrecan (AGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aggrecan (AGC) in the samples is then determined by comparing the OD of the samples to the standard curve.