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Product Description
Pig IgG (Immunoglobulin G) ELISA Kit | ELK2190 | ELK Biotechnology
Alternative Names: Immunoglobulin G
Species: Pig
Assay Type: Competitive Inhibition
Sensitivity: 0.57 μg/mL
Standard: 100 μg/mL
Detection Range: 1.57-100 μg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Infection immunity;Immune molecule;Hematology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G (IgG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G (IgG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G (IgG) in the samples is then determined by comparing the OD of the samples to the standard curve.
