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Product Description
Pig LTF (Lactoferrin) ELISA Kit | ELK6152 | ELK Biotechnology
Alternative Names: HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
Species: Pig
Assay Type: Sandwich
Sensitivity: 13.2 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lactoferrin (LTF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactoferrin (LTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactoferrin (LTF) in the samples is then determined by comparing the OD of the samples to the standard curve.
