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Product Description
Rab ST2 (Syntenin 2) ELISA Kit | ELK8312 | ELK Biotechnology
Alternative Names: SDCBP2; SITAC18; Syndecan Binding Protein 2
Species: Rabbit
Assay Type: Sandwich
Sensitivity: 0.065 ng/L
Standard: 10 ng/L
Detection Range: 0.16-10 ng/L
Sample Type: serum, plasma, and other biological fluids
Assay Lenght: 2h
Research Area: Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syntenin 2 (ST2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Syntenin 2 (ST2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syntenin 2 (ST2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syntenin 2 (ST2) in the samples is then determined by comparing the OD of the samples to the standard curve.
