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Product Description
Rabbit NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit | ELK2957 | ELK Biotechnology
Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP (1-32) ; BNP (5-29) ; BNP-32; Gamma-brain natriuretic peptide; nppb
Species: Rabbit
Assay Type: Sandwich
Sensitivity: 12.4 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Endocrinology;Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the samples is then determined by comparing the OD of the samples to the standard curve.
