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Product Description
Rat a2M (Alpha-2-Macroglobulin) ELISA Kit | ELK7473 | ELK Biotechnology
Alternative Names: α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Species: Rat
Assay Type: Sandwich
Sensitivity: 5.9 ng/mL
Standard: 800 ng/mL
Detection Range: 12.5-800 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity;Hematology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin (a2M) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin (a2M) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin (a2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin (a2M) in the samples is then determined by comparing the OD of the samples to the standard curve.
