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Product Description
Rat ALP (Alkaline Phosphatase) ELISA Kit | ELK5657 | ELK Biotechnology
Alternative Names: AKP; ALKP; Basic Phosphatase
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.53 ng/mL
Standard: 80 ng/mL
Detection Range: 1.25-80 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity;Hepatology;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase (ALP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alkaline Phosphatase (ALP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase (ALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase (ALP) in the samples is then determined by comparing the OD of the samples to the standard curve.
