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Product Description
Rat AMN (Amnionless) ELISA Kit | ELK7402 | ELK Biotechnology
Alternative Names: 5033428N14Rik; amn; Amnion associated transmembrane protein; Amnionless; Amnionless homolog; AMNLS; AV002116; PRO1028; Protein amnionless; Type I transmembrane protein; UNQ513/PRO1028; Visceral endoderm specific type 1 transmembrane protein
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.214 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Reproductive science;Developmental science;Gastroenterology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amnionless (AMN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Amnionless (AMN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amnionless (AMN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amnionless (AMN) in the samples is then determined by comparing the OD of the samples to the standard curve.
