Euro
USD
British Pound
NULL
Product Description
Rat aMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit | ELK5099 | ELK Biotechnology
Alternative Names: α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 48.1 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Tumor immunity;Endocrinology;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-Melanocyte Stimulating Hormone (aMSH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the samples is then determined by comparing the OD of the samples to the standard curve.
