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Product Description
Rat C1INH (Complement 1 Inhibitor) ELISA Kit | ELK5547 | ELK Biotechnology
Alternative Names: SERPING1; C1IN; C1-INH; C1NH; HAE1; HAE2; Serpin Peptidase Inhibitor Clade G Member 1; Angioedema Hereditary; C1 Inhibitor; C1 Esterase Inhibitor
Species: Rat
Assay Type: Sandwich
Sensitivity: 2.61 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity;Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement 1 Inhibitor (C1INH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement 1 Inhibitor (C1INH) in the samples is then determined by comparing the OD of the samples to the standard curve.
