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Product Description
Rat CaN (Calcineurin) ELISA Kit | ELK5642 | ELK Biotechnology
Alternative Names: PPP3CA; PPP3-CA; CCN1; CNA1; CALN; CALNA1; PP2B; PP2-B; Protein Phosphatase 3 (formerly 2B), Catalytic Subunit, Alpha Isoformprotein Phosphatase 2B; Calcineurin A Alpha
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcineurin (CaN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calcineurin (CaN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcineurin (CaN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcineurin (CaN) in the samples is then determined by comparing the OD of the samples to the standard curve.
