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Product Description
Rat CHRNa1 (Cholinergic Receptor, Nicotinic, Alpha 1) ELISA Kit | ELK2053 | ELK Biotechnology
Alternative Names: CHR-NA1; N-AChRA1; NAChRA1; ACHRA; ACHRD; CMS2A; FCCMS; SCCMS; Acetylcholine receptor subunit alpha; N-AChR-A1; Neuronal Acetylcholine Receptor Alpha 1, Muscle
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.122 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) in the samples is then determined by comparing the OD of the samples to the standard curve.
