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Product Description
Rat CHRNb1 (Cholinergic Receptor, Nicotinic, Beta 1) ELISA Kit | ELK7265 | ELK Biotechnology
Alternative Names: N-AChRB1; NAChRB1; CMS2A; SCCMS; ACHRB; CHRNB; CMS1D; N-AChR-B1; Neuronal Acetylcholine Receptor Beta 1; Acetylcholine receptor subunit beta
Species: Rat
Assay Type: Sandwich
Sensitivity: 32 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Neuro science;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1) in the samples is then determined by comparing the OD of the samples to the standard curve.
