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Product Description
Rat CNP (C-Type Natriuretic Peptide) ELISA Kit | ELK7873 | ELK Biotechnology
Alternative Names: ANFC; C type natriuretic peptide precursor ; CNP-53 ; CNP2 ; Natriuretic peptide C ; Natriuretic peptide precursor C ; NPPC
Species: Rat
Assay Type: Sandwich
Sensitivity: 12.4 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cardiovascular biology;Hormone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Natriuretic Peptide (CNP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C-Type Natriuretic Peptide (CNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Natriuretic Peptide (CNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Natriuretic Peptide (CNP) in the samples is then determined by comparing the OD of the samples to the standard curve.
