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Product Description
Rat EDN1 (Endothelin 1) ELISA Kit | ELK2436 | ELK Biotechnology
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1
Species: Rat
Assay Type: Sandwich
Sensitivity: 2.71 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Endocrinology;Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin 1 (EDN1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Endothelin 1 (EDN1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelin 1 (EDN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelin 1 (EDN1) in the samples is then determined by comparing the OD of the samples to the standard curve.
