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Product Description
Rat GCK (Glucokinase) ELISA Kit | ELK2776 | ELK Biotechnology
Alternative Names: GK; GLK; HHF3; HK4; HKIV; HXKP; MODY2; Hexokinase-D; Hexokinase 4; Maturity Onset Diabetes Of The Young 2
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.24 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucokinase (GCK) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucokinase (GCK) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucokinase (GCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucokinase (GCK) in the samples is then determined by comparing the OD of the samples to the standard curve.
